Mcf2chem

MCF2Chem is the first manually curated knowledge base dedicated to microbial cell factory production data. It systematically integrates 8,888 production records of 1,231 biosynthetic compounds synthesized by 590 microbial strains, providing critical metrics including titer, yield, fermentation conditions, and strain engineering strategies to accelerate green biomanufacturing.

The database reveals that bacteria dominate 60% of microbial chassis, with E. coli, S. cerevisiae, and Y. lipolytica producing 78% of compounds. Key findings include: Top product categories: Fatty acids (23%), Terpenoids (19%), Shikimates/Phenylpropanoids (17%); Emerging carbon sources: CO₂ and methanol utilized by specialized strains (e.g., Bacillus methanolicus for 60 g/L glutamic acid); Temporal trends: 20-fold increase in microbial chassis usage (2000–2022) and titer optimization for platform chemicals.

Researchers leverage MCF2Chem’s recommendation system to: Predict optimal chassis for target compounds via phylogenetic and similarity algorithms (S2C/C2S routes); Compare fermentation performance across 2,678 culture condition records; Identify underutilized strains (e.g., microalgae for fatty acid production) through interactive statistical dashboards.